Fig. 7

To investigate the mechanism of PVT1 regulating TGF-β expression (A) Knockout of PVT1 down-regulated the expression of TGF-β in FaDu cells. (B) The structural pattern of TGF-β promoter activity reporter (pGL3-TGF-β). (C) Knockout of PVT1 did not affect TGF-β promoter activity. (D) The structural pattern of TGF-β 3’UTR reporter gene (pmiR-TGF-β). (ns means P > 0.05, **P < 0.01, mean ± SD, one-way ANOVA, double-tailed unpaired t-test, n = 6/group)