Fig. 4

Immune cell subsets for enrolled cases. (A) Immune cell subsets calculated by ssGSEA using transcriptome data. Cluster analyses show the relationship about immune cell subset scores among cases. Percentage of CD4 + T cells (B), CD8 + T cells (C), and B cells (D) estimated by EPIC on transcriptome data. (E) Examples of positive immunohistochemistry (IHC) staining of CD4 (a), CD8 (b), CD20 (c), and CD21 (d) for infiltrating immune cells in tumor tissues. Percentage of CD4+ (F), CD8+ (G), CD20+ (H), and CD21+ (H) cells in the three groups. J. Examples of positive IHC staining of Ki67 (a) and bcl2 (b) in tumor cells. Percentage of ki67 (K) and bcl2 (L) positive tumor cells in the three groups