Fig. 1

scRNA-seq profiling of EOCC and LOCC samples. (A-C) t-SNE plots of 47,966 cells, grouped into 17 distinct clusters and 8 major cell types, color-coded by cluster (A), cell type (B), and group (C). (D) Bubble plots illustrating the expression levels of marker genes across the major cell types. (E) t-SNE plots displaying the expression of canonical markers for each cell type.(F) The proportion of the 8 major cell types within each group (left) and per sample (right). (G) Bubble plots showing significantly enriched KEGG pathways of DEGs between EOCC and LOCC. (H) Bar plots comparing cell-cell interaction numbers and strengths between EOCC and LOCC. (I) Circle plots depicting the differential interactions among each cell type. The line width denotes the degree of difference, with red and blue lines indicating higher levels in LOCC and EOCC, respectively