Fig. 3

Characteristics of NK/T cells in EOCC and LOCC. (A-C) t-SNE plots of NK/T cells, with color-coded by cluster (A), cell type (B), and group (C). (D) Bubble plots depicting the expression levels of marker genes across various NK/T-cell subpopulations. (E) Bar plot illustrating the relative proportions of the eight identified NK/T-cell subpopulations within each group. (F) Bubble plots displaying significantly enriched KEGG pathways of DEGs between EOCC and LOCC derived NK/T cells. (G-H) Violin plots comparing immune-related gene set scores between EOCC and LOCC in CD4 + T cells (G) and CD8 + T cells (H). (I-J) Violin plots demonstrating differential expression of immune-related molecules between EOCC and LOCC in CD4 + T cells (I) and CD8 + T cells (J). Significance levels: ***, p-value < 0.001; ****, p-value < 0.0001