Fig. 4

Microscopic view of the platysma muscle. The immunohistochemical staining enhances the lymphatic vessels (visualized as brown colored cells). A) Formalin-fixed tissue stained with podoplanin (D2-40 antibodies) permits the identification of the lymphatic vessels in the muscle (500 micron scale bar); B) Formalin-fixed tissue stained with podoplanin (D2-40 antibodies), using a 250 micron scale bar – perpendicular view; C) Formalin-fixed tissue stained with podoplanin (D2-40 antibodies) using a 100 micron scale bar – longitudinal view; D) Formalin-fixed tissue stained with podoplanin (D2-40 antibodies) enhances a blood vessel (green arrows) surrounded by lymphatic channels (brown) (250 micron scale bar)