Fig. 5

Overexpressing of TNFR1 reversed the decreased cell viability, invasion, migration, and inflammation induced by silencing EDN1 in A549/DDP cells. A, RT-qPCR was performed to detect the mRNA level of TNFR1 in A549/DDP cells after TNFR1 overexpression; B, The cell viability in each group was assessed by CCK-8 assay; Detection and quantification of cell C, invasion and D, migration in each group; The levels of E, TNF-α, F, IL-6, and G, IL-1β in A549 and A549/DDP cells were assessed using ELISA. (n = 3; **p < 0.01 and ***p < 0.001 vs. pcDNA3.1-NC group or sh-NC group; ^#p < 0.05, ^##p < 0.01, and ^###p < 0.001 vs. sh-EDN1 + pcDNA3.1-NC group). RT-qPCR, reverse transcription-quantitative polymerase chain reaction; TNF-α, tumor necrosis factor α; IL, interleukin; ELISA, enzyme-linked immunosorbent assay; CCK-8, cell counting kit-8; EDN1, endothelin 1; DDP, cisplatin; shRNA, short hairpin RNA; TNFR1, tumor necrosis factor receptor-1