Fig. 3

Network pharmacology evaluation of the potential targets of DCC-2036 for application in osteosarcoma a. The DCC-2036 SMILES notation was obtained from PubChem: (CC(C)(C)C1 = NN(C(= C1)NC(= O)NC2 = C(C = C(C = C2)OC3 = CC(= NC = C3)C(= O)NC)F)C4 = CC5 = C(C = C4)N = CC = C5). Using this SMILES notation, the potential targets of DCC-2036 were predicted on the SwissTargetPrediction website (https://swissmodel.expasy.org/). (b) Volcano plot analysis of gene expression differences is presented; red dots indicate genes with high expression, gray dots represent genes with no significant change, and blue dots indicate genes with low expression. Significance was determined at p < 0.05, with log2(Fold Change) OS vs. OB ≥ 1. (c) The heat map indicates gene expression levels, with red representing high expression and blue representing low expression. d-e. PCA (Principal Component Analysis) and UMAP (Uniform Manifold Approximation and Projection) diagrams illustrate the distribution of samples. A greater distance between samples in different groups indicates a larger difference between those groups. (d) PCA diagram and (e) UMAP diagram. f. Venn diagram illustrating the intersection between the 100 effective targets of DCC-2036 and 3,310 differentially expressed genes