Fig. 4

Illustrates the construction of a protein-protein interaction network diagram, topological analysis, expression grouping of key differentially expressed genes (DEGs), and visualization of DCC-2036 binding with these key DEGs a. The protein interaction network diagram depicts ten potential target genes. b-c. Topological analysis shows that the intensity of the red color in the target nodes correlates with their Degree value, indicating a stronger interaction capability with other targets. In contrast, targets exhibiting a more yellow color possess a lower Degree value. d. A comparison of the expression levels of HCK, MAPK14, PTK2B, KDR, and CSF1R between the control group and the OS group was performed using mean ± SD and Student’s t-test (***, p < 0.001). e. The binding energy values of HCK, MAPK14, PTK2B, KDR, CSF1R, and DCC-2036 were analyzed. f-j. The 2D docking visualization of DCC-2036 with HCK, MAPK14, PTK2B, KDR, and CSF1R is presented. k. 143B cells were treated with DCC-2036 at varying concentrations (0 µM, 2.5 µM, 5 µM, 10 µM) for 48 h, and changes in HCK/KDR phosphorylation and total protein levels were assessed using western blot analysis