Fig. 3

NMF clustering analysis classified breast cancer patients into three different subtypes based on CARGs. A NMF clustering of the expression matrix of 39 prognostic genes. Red color represented the baseline. B NMF heatmap with an optimal number of groups of 3. Red for group 1, blue for group 2, and green for group 3. C PCA plot showing the distribution of the three groups of samples in space. The patients in the three groups were distributed in different directions. D Comparison of overall survival (OS) between the three groups. E GSE20685 validation set cluster survival analysis, again the group 1 had the lowest survival rate. F Differential gene KEGG pathway maps for group 1 and group 2. G Differential gene KEGG pathway maps for group 1 and group 3. H Differentia gene KEGG pathway maps for group 2 and group 3