Fig. 2

PVT1-214 Promotes GC cell growth, invasion, migration, and colony formation. (a) SGC7901 and AGS cells were used to construct the PVT1-214 overexpression and knockdown models separately, and successful construction was validated via qRT‒PCR. (b) A CCK-8 assay was conducted to analyze cell proliferation, compared with NC transfection, PVT1-214 siRNA transfection apparently decreased GC cell viability; PVT1-214 overexpression promoted GC cell proliferation relative to that in the NC group. (c) AGS and SGC7901 GC cell invasion and migration were measured through Transwell assays. (d-e) GC cell proliferation was detected through EdU (d) and colony formation (e) assays. *P < 0.05, **P < 0.01, ***P < 0.001, respectively