Fig. 6

PVT1-214 promotes SLC45A4 expression, whereas miR-671-5p represses SLC45A4 expression. (a) The miR-671-5p downstream target was estimated on the basis of the BiBiServ database. The binding site in SLC45A4 for miR-671-5p is displayed. (b) SGC7901 cells was used to construct the SLC45A4 knockdown models, and successful construction was validated via qRT‒PCR. (c) Transwell assays were implemented to detect AGS and SGC7901 cell invasion and migration. (d) GC cell proliferation was detected through EdU. (e) qRT‒PCR and Western blotting were implemented to detect SLC45A4 expression after PVT1-214 overexpression or knockdown in GC cells. (f) qRT‒PCR and western blotting were carried out to detect SLC45A4 expression following miR-671-5p overexpression or knockdown in GC cells. *P < 0.05, **P < 0.01