Fig. 1

Anlotinib can inhibit the growth of ATC cells, block cell cycle progression at the G2/M phase, and induce apoptosis. (A and B) C643 and CAL-62 cells cultured in vitro were treated with anlotinib (0, 0.5, 1, 2, 5, 10, 20, and 40 µmol/L). After 24 h, cell viability was determined via the CCK-8 assay, and the cells were observed and images were captured under an inverted microscope. Scale bar: 100 μm. (C) C643 cells were treated with anlotinib (0.7 µmol/L) for 24 h in vitro, stained with PI, analysed by flow cytometry, and quantified via ModFit software. (D and E) C643 (D) and CAL-62 (E) cells were treated with anlotinib for 24 h in vitro, stained with Annexin V-FITC/PI, analysed by flow cytometry, and analysed via FlowJo software. Upper left quadrant (Q1): Mechanically injured cells, upper right quadrant (Q2): late apoptotic cells, lower right quadrant (Q3): early apoptotic cells, lower left quadrant (Q4): normal cells. All the data were obtained from three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001