Fig. 3

Anlotinib can inhibit the growth of subcutaneous xenografts in nude mice. Nude mice in the control group (n = 8) and the anlotinib group (n = 8) were intraperitoneally injected with equal volumes of PBS containing dimethyl sulfoxide (100 µl/d) or anlotinib diluted with PBS (6 mg/kg, 100 µl/d), respectively. After 14 days of treatment, the nude mice were sacrificed by cervical dislocation, and the tumour tissues were removed. (A) Visual comparison of the size of tumour tissues in nude mice in the control group and the anlotinib group. (B) Quantitative comparison of the weights of the tumour tissues from the nude mice in the control and anlotinib groups. Comparison of the tumour volume (C) and body weight (D) of the nude mice during drug administration. (E) HE, Ki67, and CD31 IHC staining of tumour tissues with or without anlotinib treatment. Scale bar: 100 μm. ** p < 0.01, **** p < 0.0001