Fig. 2

Differences between Gross and Pathological Diagnoses. H&E and EVG stained micrographs of the same case were shown in a vertical row. Arrow heads: elastic lamina (EL); a) b) c) Fresh resected specimens; gross diagnosis of serosal invasion was cT4a. d) e) f) H&E staining of cT4a area at low-power view. g) h) i) H&E staining of the black circle areas at high-power view. d) e) g) h) The pathological diagnosis was pT3 by H&E staining, with a discrepancy between the gross and pathological diagnoses of serosal invasion. i) Several cancer cells in the rectangle area are exposed to the serosal surface and peritoneal cavity. The pathological diagnosis was pT4a by H&E staining alone, which was compatible with the gross diagnosis. j) k) l) EVG staining of the H&E staining areas; j) Cancer cells do not invade the EL, i.e. pT3a (ELI −), k) Fragmented ELs are seen on the left. Cancer cells invade beyond the ELs, i.e. pT3b (ELI +), l) Cancer cells invade beyond the EL and several cancer cells in the rectangle area are exposed to the serosal surface and peritoneal cavity, i.e. pT4a (ELI +)